The Aussie star beat home favorite Paul Jubb 3-6 6-1 7-5 6-7 (3-7) 7-5 in an entertaining game but felt a fan overstepped the bar in his heckling.
After winning a match point, Kyrgios turned to one of the stands and spat on the ground as he headed for the net.
Asked by a reporter if he spat in the direction of this fan, Kyrgios replied, “From one of the people who disrespects me, yes. I wouldn’t do that to someone who supported me.
“I agree to receive many [heckling]. What I don’t understand is that as soon as I give it back, for example in Stuttgart, I give it back and get a game penalty.
“As soon as I won the game, I turned to him. I’ve been dealing with hate and negativity for a long time, so I don’t feel like I owe this person anything.
“He literally came to the game to not even really support anyone. It was more just to stir and disrespect.”
Kyrgios said earlier this month he was racially assaulted by a member of the crowd at a tournament in Stuttgart, which organizers said they were investigating.
The Australian said he was not racially assaulted on Tuesday but was concerned about the frequency of abuse from fans.
“I’m just starting to think it’s normal when it really isn’t,” he told reporters. “I didn’t say anything to the crowd until it started every time I went down to the bottom. I don’t know if that’s normal or not.
“It has nothing to do with Wimbledon. I just think it’s a whole generation of people, like on social media, who have the right to comment on everything with negativity. It continues in real life.
“Because there’s a fence there and I physically can’t do or say anything because I’m going to get in trouble, so they just feel like they can say whatever they want. want.”
It wasn’t the only flashpoint of the match, as Kyrgios was also unhappy with a linesman’s call on a point in the first set.
He later said at his press conference that referees at Wimbledon should be younger because “in fact, young people have better eyesight”.
Krygios is set to play doubles with Thanasi Kokkinakis on Wednesday, before continuing his singles campaign against 26th seed Filip Krajinović on Thursday.